How could I compare and contrast the Southern and Middle Colonies?

You could discuss geography and how it affected colonial populations and land use; the Middle colonies contained a more extensive system of rivers, which made trade more efficient. As such, there were larger enterprises there, including timber and mining. The Southern colonies were comparatively less urban, with scattered farms that grew more cash crops. As plantations were established, there were more slaves and indentured servants concentrated in the Southern Colonies, particularly South Carolina and Virginia.
Literacy rates were comparably higher in Middle colonies's urban areas, in which wealthy individuals could afford tutoring in reading and writing. The population demographics also differed. The middle colonies were founded by a more diverse group of Europeans, including the Dutch, Germans, Irish, and Swedish, furthered by policies of tolerance. The Anglican Church of England was established throughout the Southern Colonies, and Anglicans made up a majority of population.